Protein Analysis Reagents
Thermo Scientific™ Pierce™ Protein A Plus Agarose
Affinity purify antibodies (IgG) with densely immobilized Protein A offered in bottles, spin columns, filter plates and complete purification kits.
Thermo Scientific™ EZ-Link™ NHS-PEG Solid-Phase Biotinylation Kit - Mini-Spin Columns
A column-based approach to antibody biotinylation and clean-up
Thermo Scientific™ BMOE (bismaleimidoethane)
Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this short crosslinker composed of maleimide groups and a 7-atom (8.0A) spacer arm.
Thermo Scientific™ BS2G-d0 (bis(sulfosuccinimidyl) glutarate-d0)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of a 7.7A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientific™ MS(PEG)24 Methyl-PEG-NHS-Ester Reagent
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
Invitrogen™ eBioscience™ 10X RBC Lysis Buffer (Multi-species)
Specially formulated for optimal lysis of erythrocytes in single-cell suspensions of peripheral blood and hematopoietic tissues such as spleen. This buffer can be used for lysis of human, mouse, rat, canine, and non-human primate samples.
Invitrogen™ eBioscience™ Brefeldin A Solution (1000X)
Addition of Brefeldin A during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines.
Thermo Scientific™ BMPH (N-β-maleimidopropionic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this short (8.1A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ DyLight™ 594 NHS Ester
Label antibodies or other proteins as probes for fluorescence imaging assays with the full spectral range of NHS ester-activated DyLight Dyes.
Invitrogen™ 5-ROX (5-Carboxy-X-Rhodamine, Triethylammonium Salt), single isomer
5-ROX (5-Carboxy-X-Rhodamine, Triethylammonium Salt), single isomer
Invitrogen™ eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 570
Contains reagents and buffers to identify and examine proliferating cells by immunohistochemical or immunocytochemical analysis. Optimized for IHC with both frozen and formalin-fixed paraffin-embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.
Cytiva HiTrap™ Q Sepharose Fast Flow IEX Columns
Use for small-scale protein purifications. Cytiva Life Sciences™ HiTrap™ Q Sepharose Fast Flow IEX Columns are strong anion exchangers for rapid separations either alone or connected in series.
Cytiva Glutathione Sepharose™ 4B Media
Use for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a single-step for purification.
Cytiva HisTrap™ FF Crude Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Cytiva HisTrap™ FF Columns: 5 x 5mL
Optimize purifications of histidine-tagged proteins directly from homogenized, unclarified cells HisTrap™ FF Columns: 5 x 5mL are prepacked with precharged Ni Sepharose™ 6 Fast Flow media.